Submitted to Fish and Shellfish Immunology 5/6/05 The persistence of acquired immunity of largemouth bass Micropterus salmoides to glochidia larvae of Lampsilis reeveiana (Bivalvia, Unionidae)
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چکیده
Host fish acquire immunity to the parasitic larvae (glochidia) of freshwater mussels (Unionidae). We investigated the persistence of acquired immunity of largemouth bass, Micropterus salmoides (Lacepède, 1802) to glochidia of the broken rays mussel, Lampsilis reeveiana (Call, 1887). Fish received 3 successive priming infections with glochidia to induce an immune response. Primed fish were held at 21-23 C and were challenged (re-infected) at intervals after priming. Metamorphosis success was quantified as the percent of attached glochidia that metamorphosed to the juvenile stage and were recovered alive. Metamorphosis success at 3, 7, and 12 months after priming was significantly lower on primed fish (26%, 40%, and 68% respectively) than on control fish (85%, 93%, and 92% respectively). A second group of largemouth bass was similarly primed and blood was extracted 0, 3, 7 and 12 months after priming. Immunoblotting was used to detect host serum antibodies to L. reeveiana glochidia proteins. Serum antibodies were evident in primed fish, but diminished over time and were nearly undetectable 7 and 12 months after priming. Primed fish had antibodies present in their serum within 4 days when re-infected 7 months after priming, but fish that received their first infection did not. The results are consistent with the hypothesis that antibodies are at least part of the mechanism of acquired immunity to glochidia. Acquired immunity of host fish potentially affects natural reproduction and artificial propagation of unionids, many of which are of conservation concern.
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